Highly sensitive and specific GON 10 Mouse Monoclonal Antibody
Cat.No. BT-MCA0354 Size: 20ul/50ul/100ul Datasheet Request
Product Information
Clonallity: Monoclonal
Applications: WB, IHC, IF, ELISA
Source: mouse
Isotype: IgG
Immunogen: HDAC1 fusion protein
Synonyms: GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1
Species specificity: human, mouse, rat
Recommended Dilution:
WB : 1:20000-1:100000
IHC : 1:50-1:500
IF : 1:50-1:500
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Store at 4°C for a month, for one year store at -20°C. When reconstituted in sterile pH 7.4 0.01 M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-20°C. Avoid repeated freeze/thaw cycles.
Background
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calcualted molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .