Active Member
7 Years
Shanghai Korain Biotech Co., Ltd
Shanghai Korain Biotech Co Ltd
Manufacturer from China
Shanghai Korain Biotech Co., Ltd
Shanghai Korain Biotech Co Ltd
Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researchers all over the world. Our products line covers a set of research areas including Immunology, Neuroscience, Cancer, Kinases, Phosphatases and Cell Biology.
Bioassay Technology Laboratory (BT Lab) as a leading brand of Korainbio focused on helping researchers to optimize life science work and serves as a provider of test and developing services, including elisa tests, WB test and antigen development. Founding in 2010, our strategic focus has been on the development of enabling technologies in the research, development, manufacture and marketing of innovative immuno products and services based on molecular technologies.
Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:
ELISA and immunohistochemistry
About BT Lab
As a leading brand of Korainbio, Bioassay Technology Laboratory (BT Lab) offers a variety of cost-effective ELISA kits and sets to measure cytokines, chemokines, and soluble biomarkers consistently and reliably. Sandwich kit and Competitive that provide the core reagents our selection of ELISA products meets the demand of ELISA beginners to experts alike at a very economical price.
Worldwide Distributors
ELISA Kit Categories
Sandwich Kit
Sandwich kits are fully validated and ready-to-use, containing 96-
well strip plates pre-coated with capture antibody to detect sample
antigen.
Competitive Kit
In a competitive ELISA assay, sample antigen and labeled antigen
compete for capture antibody binding. The more target protein there
is in the sample, the less labeled antigen will be captured and the
weaker the signal.
Qualitative Kit
Qualitative results provide a simple positive or negative result
for a sample. In quantitative ELISA, the optical density (OD) of
the sample is compared to a standard curve, which is typically a
serial dilution of a known-concentration solution of the target
molecule. It is suitable for detecting small antigens.
Advantages of our Sandwich kit
Without Diluting Sample
Streptavidin-biotin system
Washing Step
Pre-Coated plate
What We Provide
What We Aim to
If you’re interested in working with us to expand the market, please feel free to contact us by: save@bt-laboratory.com
| Company Name: | Shanghai Korain Biotech Co., Ltd |
|---|---|
| Business Type: | Manufacturer,Distributor/Wholesaler,Exporter,Seller |
| Brands: | BT Lab |
| Year Established: | 2010 |
| Company Location: | #1008.Junjiang Internatioanl Bldg. 228 Ningguo Rd. Yangpu Dist. Shanghai. 200090. China |
| Factory Location: | Baoshan Dist. Shanghai. China |
Shanghai Korain Biotech Co Ltd
OEM is also available by us. We can produce as any request from customer.
Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:
Packing and Shipment
Custom Package
Ship by DHL/FedEX at our discounted rate
Techinical Laboratory Clean Breeding Environment
Sandwich/Competitive ELISA kit
Need to be provided by customers:
| Type | QTY | Time |
| High sensitivity matching selection (Materials provided by customers) | One pair | (15-20days) |
| Normal matching selection (Materials provided by customers) | One pair | (15-20days) |
| Wide detection range matching selection (Materials provided by customers) | One pair | (15-20days) |
Antibody or Antibody pair can be specifically provided as customer's request.
Developing charges: 1650USD (Not including the antibody fees)
1) Recombinant purification protein or natural purified protein 5mg(protein purity should be higher than 80%)
2) Synthetic polypeptide antigen (5mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.
3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).
4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.
Producing procedure
1. Vaccinating 5 BALB/ c strain mice with standard immune procedure. The antibody titers in serum will be determined by ELISA.
2. After the antibody titers are achieved appropriate values, the mouse spleen cells were fused to form hybridoma. Then determining the supernatant of growing hybridoma cell by ELISA.
3. Subcloning the hybridoma cell lines by the method of finite dilution, finally provide 2 hybridoma cell lines to the customer.
4. The Preparation of BALB/ c strain mice Ascites, antibody purification.
The project completion time: 4-5 months
2.Rabbit Polyclonal Antibody Production
Provided by customers：
1) Purification protein or natural purified protein 10mg (protein purity should be higher than 80%).
2) Prepare polyclonal antibody with polypeptide antigen (10mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.
3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).
4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.
Producing procedure：
Polypeptide or Protein Antigen/ Mycoprotein, etc. Immunizing 1-2 New Zealand rabbits (or 3 rats); determine the titers by collecting (rabbits) auricular arterial blood, serum were collected by bleeding of carotid artery; determine the titers by collecting (rats) tail veins blood, collected serums from heart.
Production result
Plan 1: Provide 10ml serum, titers >1: 10000. Including the report of titers value which were determined by indirect ELISA.
Plan 2: Povide 10 mg (Rabbit) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, 2 mg (Rat) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, antibody titers(1mg/ml) > 1: 5000; Including the report titers value which were determined by indirect ELISA.
Plan 3: According to the requirement, collecting serum by sustaining immunization.
The project completion time: 4-5months.
Antigen Production sevice
| Content(Unit:/each) | QTY | Purification | Time |
| Epxress the prokaryotic proteins | 10mg | >90% | 30-45days |
| Synthesized and coupled polypeptide | 5-10mg/each | >90% | 30-45days |
| Nature purity protein(Provided by customer) | 5mg | >80% | 30-45days |
Our technical team consist of professional staff and expert.
Shanghai Korain begun at 2010, and then Bioassay Technology Laboratory (BT Lab) comes out as a brand under korain; as a leading brand of Korainbio focused on helping researchers to optimize life science work and serves as a provider of test and developing services, including elisa tests, WB test and antigen development. Founding in 2010, our strategic focus has been on the development of enabling technologies in the research, development, manufacture and marketing of innovative immuno products and services based on molecular technologies.
Now BT Lab is growing stronger.
What We Treasure
Creative & Integrity
What We Aim to
What We Provide:
| Market name: | Worldwide |
|---|---|
| Contact Person: |