Shanghai Korain Biotech Co., Ltd
Shanghai Korain Biotech Co Ltd
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Rabbit Oxidized Lowdensity Lipoprotein ELISA Kit with High Spesificity and Sensitivity
Cat.No E0018Rb
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (192μg/dl) with 120μl of standard diluent to generate a 96μg/dl standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (96μg/dl) 1:2 with standard diluent to produce 48μg/dl, 24μg/dl, 12μg/dl and 6μg/dl solutions. Standard diluent serves as the zero standard(0 μg/dl). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
| 96μg/dl | Standard No.5 | 120μl Original Standard + 120μl Standard Diluent |
| 48μg/dl | Standard No.4 | 120μl Standard No.5 + 120μl Standard Diluent |
| 24μg/dl | Standard No.3 | 120μl Standard No.4 + 120μl Standard Diluent |
| 12μg/dl | Standard No.2 | 120μl Standard No.3 + 120μl Standard Diluent |
| 6μg/dl | Standard No.1 | 120μl Standard No.2 + 120μl Standard Diluent |
| Standard Concentration | Standard No.5 | Standard No.4 | Standard No.3 | Standard No.2 | Standard No.1 |
| 192μg/dl | 96μg/dl | 48μg/dl | 24μg/dl | 12μg/dl | 6μg/dl |