Shanghai Korain Biotech Co., Ltd

Shanghai Korain Biotech Co Ltd

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96 Wells 48 Wells Mouse MGP Sandwich ELISA Kit With Strong Sensitivity and Specificity

96 Wells 48 Wells Mouse MGP Sandwich ELISA Kit With Strong Sensitivity and Specificity

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Brand Name :BT Lab
Model Number :Cat.No E1801Mo
Certification :CE, ISO9001:2005, MSDS
Place of Origin :Shanghai, China
MOQ :Negotiation
Price :Negotiation
Supply Ability :Western Union, T/T
Delivery Time :1-3 business days, bulk order within one week
Packaging Details :Wrapped with ice pack and styrofoam package
Storage :2-8°C
Lead Time :Within 48 hours
Bulk Order :Yes
Discount :Available
Quality :CE, ISO
Shipping :DHL/FedEX
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96 Wells 48 Wells Mouse MGP Sandwich ELISA Kit With Strong Sensitivity and Specificity

Cat.No E1801Mo
Standard Curve Range: 5ng/L - 2000ng/L
Sensitivity: 3.09ng/L
Size: 96 wells
Intended Use
This sandwich kit is for the accurate quantitative detection of Mouse Matrix Gla Protein (also known as MGP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse MGP antibody. MGP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MGP Antibody is added and binds to MGP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MGP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MGP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.


Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

Tissue other body fluids Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.

Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (2400ng/L) with 120μl of standard diluent to generate a 1200ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (1200ng/L) 1:2 with standard diluent to produce 600ng/L, 300ng/L, 150ng/L and 75ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

1200ng/LStandard No.5120μl Original Standard + 120μl Standard Diluent
600ng/LStandard No.4120μl Standard No.5 + 120μl Standard Diluent
300ng/LStandard No.3120μl Standard No.4 + 120μl Standard Diluent
150ng/LStandard No.2120μl Standard No.3 + 120μl Standard Diluent
75ng/LStandard No.1120μl Standard No.2 + 120μl Standard Diluent

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
2400ng/L1200ng/L600ng/L300ng/L150ng/L75ng/L


Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.

Calculation of Result
Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

Referances
"Matrix GLA protein, an inhibitory morphogen in pulmonary vascular development."
Yao Y., Nowak S., Yochelis A., Garfinkel A., Bostrom K.I.
J. Biol. Chem. 282:30131-30142(2007)














































China 96 Wells 48 Wells Mouse MGP Sandwich ELISA Kit With Strong Sensitivity and Specificity for sale

96 Wells 48 Wells Mouse MGP Sandwich ELISA Kit With Strong Sensitivity and Specificity

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