Products | Suppliers | 登录 | 注册 | 外贸论坛
Shanghai Korain Biotech Co., Ltd
Shanghai Korain Biotech Co Ltd
Verified Supplier

1 Years

Home > Products > Mouse ELISA Kit >

96 Wells Mouse High Precision Endothelin 1 ET 1 Sandwich ELISA Kit

Shanghai Korain Biotech Co., Ltd

City: shanghai

Province/State:shanghai

Country/Region:china

View Contact Details

96 Wells Mouse High Precision Endothelin 1 ET 1 Sandwich ELISA Kit

Brand Name : BT Lab
Model Number : Cat.No E0257Mo
Certification : CE, ISO9001:2005, MSDS
Place of Origin : Shanghai, China
MOQ : Negotiation
Price : Negotiation
Payment Terms : Western Union, T/T
Supply Ability : In Stock
Delivery Time : 1-3 business days, bulk order within one week
Packaging Details : Wrapped with ice pack and styrofoam package
Delivery : Within 48 hours
Sensitivity : 0.43ng/L
Standard Curve Range : 1ng/L - 400ng/L
Shipping : DHL/FedEX
Size : 96 wells/48 wells
Quality : CE, ISO
Contact Now

96 Wells Mouse High Precision Endothelin 1 ET 1 Sandwich ELISA Kit
​​

Cat.No E0257Mo

Standard Curve Range: 1ng/L - 400ng/L

Sensitivity: 0.43ng/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%


Intended Use

This sandwich kit is for the accurate quantitative detection of Mouse Endothelin 1 (also known as ET-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.


Reagent Provided

ComponentsQuantity
Standard Solution (480ng/L)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Mouse ET-1 Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


Note

  • Sample concentrations should be predicted before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their particular experiments.
  • Samples to be used within 5 days should be stored at 2-8°C. Samples should be aliquoted or must be stored at -20°C within 1 month or -80°C within 6 months. Avoid repeated freeze thaw cycles.
  • Samples should be brought to room temperature before starting the assay.
  • Centrifuge to collect sample before use.
  • Samples containing NaN3 can’t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).
  • Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
  • Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.

*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.


Reagent PreparationAll reagents should be brought to room temperature before use.Standard Reconstitute the 120μl of the standard (480ng/L) with 120μl of standard diluent to generate a 240ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (240ng/L) 1:2 with standard diluent to produce 120ng/L, 60ng/L, 30ng/L and 15ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20℃ and used within one month. Dilution of standard solutions suggested are as follows:


240ng/LStandard No.5120μl Original Standard + 120μl Standard Diluent
120ng/LStandard No.4120μl Standard No.5 + 120μl Standard Diluent
60ng/LStandard No.3120μl Standard No.4 + 120μl Standard Diluent
30ng/LStandard No.2120μl Standard No.3 + 120μl Standard Diluent
15ng/LStandard No.1120μl Standard No.2 + 120μl Standard Diluent

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
480ng/L240ng/L120ng/L60ng/L30ng/L15ng/L

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


Assay Procedure

1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

4. Add 40μl sample to sample wells and then add 10μl anti-CAMP/LL-37 antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells ( Not blank control well ). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.


Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.


Product Tags:

rat elisa kit

      

sandwich elisa kit

      
China Customized Inflatable Bumper Ball Game Bubble Adult Grass CE supplier

96 Wells Mouse High Precision Endothelin 1 ET 1 Sandwich ELISA Kit Images

Send your message to this supplier
From:
Enter your email please.
To: Shanghai Korain Biotech Co., Ltd
Subject:
Message:
Characters Remaining: (0/3000)
 

Français| Русский язык| Español| Português| 日本語

Home| Products| Suppliers| Site Map| About Us| Contact Us| Help| 关于我们| 联系我们

Copyright © 2009 - 2017 everychina.com. All rights reserved.

Inquiry Cart 0