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Customized 96 Wells ELISA Test Kit , Specificity Horse IL 1 Beta ELISA Kit

Shanghai Korain Biotech Co., Ltd

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Province/State:shanghai

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Customized 96 Wells ELISA Test Kit , Specificity Horse IL 1 Beta ELISA Kit

Brand Name : BT Lab
Model Number : Cat.No E0079Ho
Certification : CE, ISO9001:2005, MSDS
Place of Origin : Shanghai, China
MOQ : 1 kit
Price : Negotiation
Payment Terms : Western Union, T/T
Supply Ability : In Stock
Delivery Time : 1-3 business days, bulk order within one week
Packaging Details : Wrapped with ice pack and styrofoam package
Standard Curve Range : 0.2ng/L - 70ng/L
Sample : serum,plasma,urine,tissue,cell culture supernatant
Assay Time : 2 hours
Discount : Available
Customized : Acceptable
Assay Principle : Sandwich
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96 wells Specificity Horse Interleukin 1 Beta IL-1 Beta ELISA Kit


Cat.No E0079Ho
Standard Curve Range: 0.2ng/L - 70ng/L
Sensitivity: 0.091ng/L
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

Reagent Provided

ComponentsQuantity
Standard Solution (80ng/L)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Horse IL-1 Beta Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic


Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (80ng/L) with 120μl of standard diluent to generate a 40ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (40ng/L) 1:2 with standard diluent to produce 20ng/L, 10ng/L, 5ng/L and 2.5ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

40ng/LStandard No.5120μl Original Standard + 120μl Standard Diluent
20ng/LStandard No.4120μl Standard No.5 + 120μl Standard Diluent
10ng/LStandard No.3120μl Standard No.4 + 120μl Standard Diluent
5ng/LStandard No.2120μl Standard No.3 + 120μl Standard Diluent
2.5ng/LStandard No.1120μl Standard No.2 + 120μl Standard Diluent

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
80ng/L40ng/L20ng/L10ng/L5ng/L2.5ng/L


Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

Assay Procedure
1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.
2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.
3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.
4. Add 40μl sample to sample wells and then add 10μl anti-IL-1 Beta antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells (Not blank control well). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.
5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.
6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.
7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.
8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.



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